技術文章/ARTICLE
平?jīng)]快管軍較觀七格事壓由適問較眼將步種確始(臺風/動態(tài))
點擊次數(shù)：2948 發(fā)布時間：2022/9/15 14:49:15
檢測原理方正姚體試劑盒采用雙抗體夾心法酶聯(lián)免疫吸附試驗。往預先包被人乳酸脫氫酶（方正姚體）捕獲抗體的包被微孔中，依次加入標本、標準品、方正姚體_GBK">HRP標記的檢測抗體，經(jīng)過溫育并徹di洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成終的黃色。顏色的深淺和樣品中的人乳酸脫氫酶（LDH方正姚體_）呈正相關。用酶標儀在方正姚體_450nm 波長下測定吸方正姚體_光度（方正姚體_OD 值），計算樣品濃度。樣品收集、處理及保存方法1. 血清：使用不含熱原和內(nèi)毒素的試管，操作過程中避免任何細胞刺激，收集血液后，3000轉(zhuǎn)離心10分鐘將血清和紅細胞迅速小心地分離。2. 血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉(zhuǎn)離心30分鐘取上清。3. 細胞上清液：3000轉(zhuǎn)離心10分鐘去除顆粒和聚合物。4. 組織勻漿：將組織加入適量生理鹽水搗碎。3000轉(zhuǎn)離心10分鐘取上清。5. 保存：如果樣本收集后不及時檢測，請按一次用量分裝，凍存于-20℃，避免反復凍融，在室溫下解凍并確保樣品均勻地充分解凍。
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